Selective Plane Illumination Microscopy (SPIM) is a fluorescence microscopy method. Only a thin layer is illuminated in the sample (usually only a few micrometers). Compared to other fluorescence microscopy methods, such as confocal microscopy, the light sheet microscope achieves significantly reduced phototoxicity and reduction in the fading of optical markers, so that biological processes can be observed over a long period of time. In addition to the necessary electronic and optical developments, work is also being done on optimized assays and image analysis routines.